FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249892
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8200.035.117258
GTCTCGC9350.034.8639371
GTATAAT6050.028.7363361
TCTCGCT10850.028.535182
TCGCTCT9900.028.3642524
GTGTATA12300.027.6801381
GTACGGT1050.00797442927.4291633
GTATTAT7400.027.4095441
GTCTTAC8200.027.3856681
GTCTTGC19750.026.0415441
GTGCTAC7650.025.5668871
CCTATCG1450.001278419724.8242598
CTCGCTC11400.024.632113
CGATCAG2051.3623336E-524.5853525
GTATTAG7800.024.1465021
GTGTAAC6550.023.224881
GTCTAAG7250.022.980811
CTGTCGC12750.022.5852099
TTATACT7100.022.3103434
GTCCCGA3254.0310624E-822.289081
Produced by FastQC (version 0.11.5)