FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249892
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9750.032.1977231
TCTGTCG9250.029.5685868
GCGTAGT2552.8103386E-928.6299841
GTCTTGC18800.027.5715871
TCGCTCT11000.026.172574
GTACCGG2351.2533146E-624.8532621
GTATTGG6850.024.5131191
GGCGTAA2151.7691511E-523.7695431
GTACGGG2802.1929009E-723.466361
TCTCGCT12650.023.3349862
GTATTAA7200.023.3215081
GTAGTAC3803.9835868E-1022.728813
GTCTTAC9700.022.5793151
CTCGCTC12550.022.3665983
GTATTAG8600.022.0717181
TAGTACT5250.021.9351064
GTACTAT5001.8189894E-1221.9019371
CGCTCTG11600.021.7164635
GTGTAAA16300.021.4988351
GTGTTAG15300.021.4724861
Produced by FastQC (version 0.11.5)