FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424302
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9850.036.7591171
TCTGTCG10600.035.317218
TCGCTCT10100.028.5178744
CTGTCGC13550.028.1595389
GTCTTGC19450.026.062121
TCTCGCT11450.025.7843912
GTTACGA1450.001235318424.9708481
CTCGCTC11200.024.4311623
GTCTCAC25500.024.422471
GTCTTAC9550.024.2648661
CGCTCTG12100.023.8036845
GGGTAAC8550.023.7150021
CTGTTGC22500.023.6776249
TAATACT8250.022.6933144
GTATAAC4505.456968E-1222.5292551
GTGTTAG14400.022.1269451
TCACTCT30100.022.0089784
GTCGTGG4307.2759576E-1121.8930471
GTGTAAG13800.021.5147381
ACCGTGT6800.021.1743478
Produced by FastQC (version 0.11.5)