FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424302
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10500.034.7683031
TCGCTCT9800.032.3129274
TCTGTCG10600.031.9143838
GTCTTGC21100.029.4129941
CTCGCTC11500.027.5362343
GTATTAA6900.026.4541441
CGCTCTG12250.025.8503425
GTATTAG8500.025.7694471
TCTCGCT13200.025.0912192
GTATTAC5550.024.995591
GTGTTAG15800.024.491851
GTGTAAG12350.023.6480751
GTCTTAC8300.022.871681
GTATAAA8750.022.529861
GTCCGGT1952.494229E-422.4656721
GTATAAC3955.4023985E-1022.1812971
GTATAGT5600.022.1647931
GTGTTAC8900.022.1501431
CGTAAAC1952.749442E-422.1445223
TTATACT6900.021.903824
Produced by FastQC (version 0.11.5)