FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527356
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9850.032.890298
GTCTCGC11650.031.0704781
GTATTAT7500.030.88821
GTACCGA1255.200282E-428.957691
GTATTAA6800.026.6155221
TCGCTCT12800.026.4407524
GGTATAC5550.026.0880071
GTCTTGC23000.025.4953561
TACACCG1701.0808278E-425.4143875
CTGTCGC13650.025.3162849
GTATAGA7900.024.7423291
GTATTAG9650.024.0063741
GTACTAG4850.023.882631
TCTCGCT13950.023.7448542
CGCTCTG14050.023.5753865
GTTACGG1851.8499563E-423.4792061
GTCTTAC10350.023.0822161
CTCGCTC13800.022.9593473
TTACACG2252.7966638E-522.4027564
GTATAAC4701.0913936E-1121.5642361
Produced by FastQC (version 0.11.5)