FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310009
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTCG700.001094465441.1357158
TCTGTCG9100.039.553578
GTCTCGC9100.033.410861
GTCTTGC20550.032.760521
TCGCTCT11250.029.4474
CTGTCGC12900.027.902139
CGCTCTG10600.027.8550745
GCGTTAA1306.5432634E-427.8423791
TCCGACG1050.00798207327.423818
GTATAAT6600.026.3237061
GTATTAC5250.026.1983551
TCTCGCT11600.026.0751632
GTATTAG8750.025.6468121
TTATACT6750.025.6060874
TACTGCG2259.665055E-725.6055347
GGTATAC4850.025.373881
GTAGCGC2301.1703742E-625.0494333
GTATTCG1801.5368446E-424.1300621
CTCGCTC12650.023.3415153
GTCCGGG5700.022.860061
Produced by FastQC (version 0.11.5)