FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361148
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8350.040.5222748
GTCTCGC11200.038.7722551
TCGCTCT10400.033.9234774
TCTCGCT12000.031.2003652
CTCGCTC12550.029.8330213
CTGTCGC12050.028.6771939
GTCTTGC20900.028.3958851
GTCTTAC9350.027.09221
CGCTCTG14250.025.7687245
GTGTTAG14650.025.195351
GTACTAT5750.023.9151761
GTATTAT7700.023.4983371
CCGTACA1851.921145E-423.348669
GTATAGC5550.022.1688821
GTTTCAC36350.021.9865043
GGGTTTC41900.021.7642841
GTACACG3004.636786E-721.7124611
GTATAGA7100.021.4066541
GTCCGGT1750.003696993720.6785351
TATAGTG7350.020.571675
Produced by FastQC (version 0.11.5)