FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361148
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC20800.031.2143331
TCTGTCG10750.030.1298758
GTCTCGC10100.026.0021081
GTGTTAC9250.024.4482271
GTACTAC3601.70985E-1024.3167861
TTGCTCT29750.023.4661184
GTAACGC1550.001887976323.2163943
GTCTCAC24050.023.052921
AGTTCGC1600.002269534422.492798
TAATAGT7050.022.458984
CTCGCTC12200.022.4171413
TCGCTCT10650.022.300824
TACTACG1650.002708931221.817662
GTACTAG4751.0913936E-1121.501161
GGTTAAC5900.021.0195941
GTCTAGC10250.020.6396141
GTCTTAC8850.020.6074471
GTATTAA7450.020.5631891
GTATAGA8950.020.3771951
GTGTTAG16500.020.3376771
Produced by FastQC (version 0.11.5)