FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402334
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9800.033.7845848
GTCTCGC8800.026.535931
TCGCTCT10950.025.634174
GTCTTGC19700.025.5593531
TAATACT7100.024.3288544
GTATTCG1801.4553829E-424.3246041
CTCGCTC11500.023.7823353
GTATAGT6500.023.5761551
TCTCGCT12200.022.4261782
CGCTCTG12700.022.10195
TTACACT12100.022.008234
GTATTAA6700.021.7832261
GTCTCAC25250.021.675391
GTATAAA8800.021.5604441
GGCTAGA13250.021.4790841
GTGTAAC7150.021.4328671
GTATAAC3757.214112E-921.4056511
GGTATCG1750.003524602620.849661
GTCTTAA8600.020.3647821
GTGTTAC10050.020.331011
Produced by FastQC (version 0.11.5)