FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975310
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAC750.001530527838.423113
GTCTCGC7600.037.215661
TCTGTCG8350.034.4873128
GTTCGGA1102.4325012E-432.9649161
GTACTAT5150.030.9806231
TCGCTCT9850.030.7010974
CTGTCGC9400.030.6350089
TCTCGCT10850.029.2194292
GTCTTGC16650.028.7477861
CGCTCTG11100.028.5360325
CTCGCTC10700.028.2786923
GTCTTAC8700.026.675061
GTATTAG6550.026.573251
GTACGGT1409.979684E-425.9010091
GTATTGC4800.025.6851671
TATTGCG1450.00127816624.824927
TACGTTG1450.001278543324.8236645
GGTATAC3753.074092E-1023.2073041
GTACACG1952.6104218E-422.3147141
TACACTG12050.021.5069735
Produced by FastQC (version 0.11.5)