FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975310
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9050.038.974688
GTCTCGC7700.032.9048421
TCGCTCT8600.029.2979774
TAATACT5500.028.7957274
GTCTTGC19800.028.1519221
CTGTCGC12000.027.593819
CTCGCTC9900.026.1785983
CGCTCTG10200.026.1137715
TCTCGCT10000.025.19692
GTATAAT6900.025.1793581
GTCTTAC8750.024.8196531
GTCTCAC24950.024.0818611
GTATTAA6300.022.981161
GTGTTAG14300.022.7802751
GTTAGCC9650.022.3806653
GTATAGA7150.022.2740461
GTACTAT5300.021.8537831
GTATTAT7300.021.816361
TACTAGG6300.021.7116132
TTGCTCT25350.021.5825964
Produced by FastQC (version 0.11.5)