FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741301
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8850.037.6318861
TCTGTCG7550.037.1841288
GTCTTGC14600.034.7125551
GTCTTAC6450.033.674641
TCGCTCT9150.030.6855134
CTGTCGC9900.030.538959
GTATTAC3450.029.3799021
GTTCCGC1000.00611658128.9601881
TCGCGCG1000.006291131528.7938639
GTACTAA3900.027.8463381
GTATTAA4100.026.4879761
CTCGCTC10600.025.809533
GTATTAT5050.025.806111
GTATTAG5900.025.769661
CTTGTCG1400.001042245725.7088099
GTGTAAT5950.025.5531081
ACTGTCG1751.3185828E-424.6804568
CGCTCTG10400.024.228395
GTATTGG4800.024.1334921
TAATACG1500.001557067323.9976464
Produced by FastQC (version 0.11.5)