FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966437
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8450.040.0426528
CTGTCGC9950.034.0060779
TCGCTCT8150.030.0424654
GTCTTGC17500.029.4265081
GTCTCGC8750.029.012051
GTGTTAC6750.026.8630081
GTGTAAG10600.026.6856141
TCTCGCT9750.026.6065392
GTCCTAT4750.025.958151
GTATAGC3108.54925E-1025.7364981
GTATTAG7100.025.5387761
GTATTAC3751.0913936E-1125.1437761
GTTTCAC28950.025.1373273
GTACGTA1450.001223893825.010391
GTACCGC1751.2527856E-424.8674721
TACGTAC1450.001271995624.8455333
GTGTAGT8200.024.7663841
GTATTAA4750.024.4312021
TTGCTCT23350.024.055924
ACCGTTC1801.5953775E-423.9971968
Produced by FastQC (version 0.11.5)