FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953543
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6550.036.2680858
GTCTCGC8350.033.88181
TCGCTCT8750.031.2683774
CTGTCGC9250.028.0164289
GTCTTAC7450.027.2640341
GTCTTGC17950.027.0768871
CTCGCTC10450.026.1923853
GTATTAT5850.026.0406481
TCTCGCT10600.025.824382
GTGTAGG9050.025.6501431
CGCTCTG10450.024.7992295
CGTTCCG1751.3184157E-424.6811395
GTCTACG1851.8243869E-423.5270751
GTGTAAG9600.023.4249571
GTCCTAC5300.023.2681271
GTGTTAG13250.022.44691
GTATAGA6250.022.052711
GGTATAC4006.85759E-1021.7625431
GTTAGCC10650.021.6425323
CGTAGTG2003.2443038E-421.6087173
Produced by FastQC (version 0.11.5)