FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953543
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9100.035.7894821
TCTGTCG7250.029.7839478
TCGCTCT10650.027.7119184
GTCTTGC15850.027.397211
GTATTAG6500.026.7228151
CTCGCTC11150.026.4719453
GTCTTAC7000.025.8479611
TCTCGCT11850.024.9113882
CTGTCGC9350.024.6341369
GTATAAT5200.023.6608281
TACCCGT1851.9224799E-423.3459685
GTGTAAC5000.023.1597751
TAATACT6050.022.6063584
GTCTCAC19950.022.4922621
CGCTCTG11300.022.2957445
GTGTAAA10750.022.2172241
GTCTAAT5900.022.0802921
GTGTTAG13600.021.818721
GTATTAC5101.8189894E-1221.2865561
TAGTACT4401.07320375E-1021.2678224
Produced by FastQC (version 0.11.5)