FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240345
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5150.043.3314938
GGATACG750.001475780638.707841
CTGTCGC6550.034.06989
GTTATAA1956.9230737E-933.497171
GTCTCGC5450.030.6289121
GTATAAG2752.1100277E-1029.0308821
GTCTTGC10800.028.2244661
TCGCTCT6200.026.709944
GTTATAC1958.68459E-626.0533541
CTTGTCG1701.0825608E-425.406899
GTTATGC2901.1777956E-825.0266231
AAACCGG1500.001557664823.9953985
GTGTAGG4353.6379788E-1223.358181
CTCGCTC6750.022.4119683
TCTCGCT6650.021.6700762
TAACACT4359.276846E-1121.517454
GTGTAAC3055.2878204E-721.4162241
CAGTCGC1700.003223456721.172419
GTGTATA3106.1827996E-721.07081
AACTGCG2104.5270356E-420.5674847
Produced by FastQC (version 0.11.5)