FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270993
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC5500.038.234171
TCTGTCG4700.035.228258
TCGCTCT6550.032.974274
GTCTTGC10850.032.0795171
TCTCGCT6700.030.1047382
CTTGTCG1753.989784E-628.7952639
CTCGCTC7200.028.0119273
CTGTCGC6850.027.3239739
GTACGGA1100.00966402426.3683931
CGCTCTG7200.024.9949055
GTATTTA3201.2387318E-924.9263731
GTGTTAG8750.023.2041851
GCTAGTC3753.3469405E-1023.051632
GTCTTAA3854.274625E-1022.6014771
TAATACT3854.674803E-1022.4396324
GGGTAAG4900.022.1978821
GTGTTGC6300.021.8690221
TTGCTCT16250.021.7089084
CGTAATC2003.242951E-421.6091983
GTGTAAC4058.039933E-1021.4853551
Produced by FastQC (version 0.11.5)