FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1628660
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6400.043.8688358
GTCTCGC7400.041.0519641
GTCTTGC13900.039.0267871
CTGTCGC8750.032.909669
TCTCGCT9050.032.6182062
GTCGTAC900.003670935332.146511
TCGCTCT9400.030.6349354
CTCGCTC9850.028.5044843
GTCTCAC18900.026.0233651
TTGCTCT21450.024.8364334
GTCTTAC5550.024.7615011
TATACTG4950.024.7245565
GTGTAAT5950.024.3124871
CGCTCTG9700.023.749975
TCTGTCA20950.023.7102748
GTCTAAG4650.023.3321461
TCTTGCT22850.023.3168752
TACTGCG2202.3544599E-522.9065727
GTATAAT4505.456968E-1222.5025561
GTCTAGT6450.022.4277971
Produced by FastQC (version 0.11.5)