FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425173
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT17830.12510761851368218No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG750.00153586638.3951645
GTCTCGC6000.037.4872931
GCGTTGG2154.838512E-1033.7469941
TCGCTCT6450.032.3759654
TCTGTCG5350.032.2961278
GTCTTGC12450.031.4700931
TCTCGCT6950.030.0657542
GTATAAG4100.028.3145541
GTATTAT3700.027.453641
GTATTAG4150.026.2250771
GTATTAA3900.026.045761
CTGTCGC7300.025.6415069
GTCCTAC4300.025.3102471
CTCGCTC7550.024.8124713
GGGTTAT3251.4733814E-924.557431
GGTTAAT3601.8371793E-1024.1853471
CCGCTCG1851.9203062E-423.349239
GTATTGT3152.843808E-823.0336651
GTCTCAC14700.022.7046131
GTATAAC2953.847981E-722.1357421
Produced by FastQC (version 0.11.5)