FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425173
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6100.035.611081
TCTGTCG6550.030.774368
TCGCTCT7150.028.1928834
GTCTTGC12000.027.7568591
TAGCGCT1306.75613E-427.689444
GTCGGAA1855.8753412E-627.3980771
GTCTTAC5450.026.5721841
GTCTCAC15900.025.502611
GTCTAGC6050.025.1337741
GCGCTAC1450.001235689624.968691
CTGTCGC8150.024.7327679
GTTTAGC3902.0008883E-1124.13641
CTCGCTC8250.023.5611973
ACTCGCC2151.97069E-523.4394323
GTGTTAG8050.023.3868241
GTACAAC2802.3725624E-723.2743851
AGGGTCG2202.354079E-522.906725
TCTCGCT8550.022.737682
CGCTCTG8100.022.219925
TACCCTT4609.094947E-1221.9107745
Produced by FastQC (version 0.11.5)