FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497496
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.043.6703538
GTCTCGC10900.041.5326161
TCGCTCT13300.034.642854
CTGTCGC14400.031.9965179
GTCTTGC22000.030.2024941
TCTCGCT13700.028.9042822
GTGTAAC8200.028.4944461
CGCTCTG14700.027.915335
GTATTAA8550.026.4740051
CCGTGTT9000.026.3971319
CTCGCTC15800.025.9718593
TTCACCG7900.025.5162125
GTTTCAC38800.024.8632763
GTATTAG9650.023.4562441
ACCGTGT9000.023.1960858
GTGTAAT10500.022.9482061
GTGTTAG17850.022.9072991
GTACTAT6100.022.7430061
TTGCTCT32950.022.5043814
GTATAGA8450.022.4667741
Produced by FastQC (version 0.11.5)