FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528816
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8200.051.8009348
GTCTCGC9950.041.4356161
TCGCTCT9650.041.054254
CTGTCGC11450.039.61279
GTCTTGC24850.035.2193221
TCTCGCT12150.032.026692
CTCGCTC11950.031.347373
CGCTCTG12400.026.7076425
GTATAGA8750.026.4523321
TTGCTCT30600.026.3644874
GTGTTAG17100.026.2251281
TATACTG7150.026.1798235
GTGTAAT11100.026.0650791
GTATTAG7800.025.0375161
TAATACT8300.024.2997994
GTGTTAC11200.023.894931
CTTGCTC29850.023.8922213
TTCACCG6350.023.809215
GTCTTAC10100.023.6327691
GTTTCAC38000.023.5073553
Produced by FastQC (version 0.11.5)