FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528816
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11250.045.4347958
GTCTCGC12550.040.1713181
TCGCTCT12450.039.3207974
CTGTCGC14350.034.616939
CTCGCTC13900.034.182463
CGCTCTG14850.031.9963345
GTCTTGC23350.030.9784031
TCTCGCT15150.030.8912182
TAATACG1652.5230074E-630.5419564
GTCTTAC11100.029.6210371
GTTTCAC36550.027.771923
GTATTAA6600.027.676221
GTATTAC7000.027.1385121
GTATTAT8700.026.0347351
CTGTTGC24950.025.6809339
CTTTGCG3109.331416E-1025.5459679
TAATACT8000.025.1971154
GTAATAC5800.024.8242513
GGGTTTC40750.024.7435591
GTATAAC5700.024.3550741
Produced by FastQC (version 0.11.5)