FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529210
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10950.042.079968
TAATACT7750.038.1063844
GTCTCGC11900.037.0506321
CTGTCGC13900.036.775039
TCGCTCT11900.035.7125324
CGTTTCG1402.882409E-530.8555079
GTATTAT8650.030.0814761
GTATTAG9900.029.9337711
TCTCGCT14400.029.5206132
CGCTCTG14500.028.3025075
CTCGCTC14550.027.227973
GTCTTGC26050.027.1913721
ATAATAC7150.026.192863
GTGTTAG16850.025.3084131
GTGTATA15200.025.2025851
CTAATAC9750.025.1182793
GTATTAC7000.024.7814081
GTGTTAC11700.024.7108061
GTACTAT6200.024.4816321
GTATAAT7000.023.748851
Produced by FastQC (version 0.11.5)