FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295388
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8600.036.8338368
GTCTCGC10000.034.7034951
TCGCTCT11100.032.4443054
GTATTCG1352.1844862E-532.1328621
CTGTCGC10550.032.0728959
GTATAGA7150.030.3352221
CTCGCTC11400.029.6989613
GTCTTGC21650.029.3870981
TACGCTA1306.756059E-427.6903765
TCTCGCT12950.027.2627222
TTCACCG7600.026.5244675
GTATTAT8200.026.4508321
GTTTCAC37400.026.1948283
GTATCGA1658.833339E-526.1799936
GGGTTTC40700.025.7576081
GTGTTAC11300.025.5925451
CGCTCTG12100.025.5849935
GTATAAC5100.025.5172731
GGTATAC5450.025.2051351
GTGTAAC8400.024.960351
Produced by FastQC (version 0.11.5)