FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295388
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9000.041.5941058
GTCTCGC9750.035.9748271
TCGCTCT11050.035.182834
TCTCGCT11900.030.2550622
CTGTCGC11600.029.7914759
CGCTCTG12100.029.1535285
GTCTTGC22300.028.8363271
GGTATAC5950.028.247041
GTACTAC3700.027.649571
CTCGCTC12600.027.4264763
GTGTTAG15050.026.2191941
GTCTTAC9650.025.7462331
GTTAGCC12050.025.093523
CCGTGTT6850.024.1738629
GTTTCAC35050.023.8269943
GTGTAGT13500.023.8166681
GGGTACG2151.7565791E-523.791491
GTATTAT8300.023.7710171
GTACTAT6100.022.7607141
TTGCTCT31350.022.7351064
Produced by FastQC (version 0.11.5)