FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780503
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.048.204838
GTCTCGC11950.038.124451
CTGTCGC14700.037.711269
TCGCTCT13600.033.368274
GTCGTAC900.00367491732.140191
TCTCGCT14150.032.0828132
GGTATAC6850.029.5595911
GTGTTAG18350.029.5567151
CTCGCTC15150.029.0065523
GTCTTGC28350.028.3139761
CGCTCTG15300.028.233545
GTCTTAC11550.027.5487351
GTATAGA6750.026.7834911
GTATTAC6700.025.9040341
GTATTAT7550.025.8611471
GTGTAAG13350.025.4593641
GTCTCAC34350.024.6314541
GTATTAG9400.024.6180171
GGGTTTC44700.024.2669221
TCACTCT39900.023.8304964
Produced by FastQC (version 0.11.5)