FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780503
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13350.047.5901831
TCTGTCG11650.045.1104748
TCGCTCT13200.037.631854
TCTCGCT16250.034.56272
CTGTCGC16500.033.162059
CTCGCTC16650.032.8609283
GTCTTGC24700.031.339231
TACACCG2851.0913936E-1130.312165
CGCTCTG15700.027.9711725
GTATTAG8650.027.859751
GTACTAA5250.026.4285721
TTCACCG9100.025.3156475
GTGTAAC8500.024.9148621
GTTTCAC41250.024.0843893
GTGTAAA15750.023.6466181
GTGTTAG18400.023.0191671
GTATTAC5850.022.4696371
GGGTTTC47050.022.3502431
GTATTAA7600.022.1000691
CTGTTGC29050.022.0574679
Produced by FastQC (version 0.11.5)