FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768353
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12000.042.5967988
GTCTCGC12800.038.9743041
TCGCTCT12750.037.844074
CTGTCGC15650.036.802359
TCTCGCT14050.033.3299832
GTCTTGC29200.032.683631
CTCGCTC14950.030.8326783
GTATTAC6600.029.5773331
CGCTCTG15850.029.0708965
CGTTTCG1306.756628E-427.6902269
TACCCGT2105.305683E-727.4270065
GTATTAG9650.026.2228551
TTAAACG2259.664873E-725.6059384
GTATAGA8600.024.3802851
TTGCTCT35850.024.3068944
GTATAAT9100.023.8352171
GTATTAA8500.023.816521
GTTTCAC42150.023.5805533
TATACTG8900.023.4593355
GTCTTAC12050.023.4000511
Produced by FastQC (version 0.11.5)