FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768353
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.049.5769128
CTGTCGC13950.040.7690939
GTCTTGC24300.040.574711
GTCTCGC10000.037.2475851
TCGCTCT11300.036.313394
TAGCGCG900.003756721731.9959989
GCGTAAC1203.888855E-430.4310341
CTCGCTC13350.029.6586783
CGCTCTG13950.029.4151465
GTGTTAG18500.028.819011
TCTCGCT13550.027.6300352
GTCTCAC32950.025.7116811
GTGTAAT12150.024.0442731
TTGCTCT36300.023.9965654
GGGTTTC46000.023.9743611
GTGTATA15000.023.8579271
GTTTCAC42000.023.6537593
GTGTAAG15300.023.3901251
GTGTTAC12150.022.842061
GTACCGT1600.00226729422.496786
Produced by FastQC (version 0.11.5)