FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821845
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.041.5637058
GTCTCGC12950.041.336621
TCGCTCT13600.034.428554
TCTCGCT15450.033.581082
CTGTCGC12600.033.1402249
CGCTCTG14650.030.4691075
GTGTTAC11750.029.5512851
CTCGCTC16350.029.0814933
GTATTAT8200.028.2298871
GTCTTGC24400.027.8683361
GTATAGC5200.026.431591
GTTACCC8450.024.7247433
GTGTAGT13150.024.2047121
GTGTTAG18700.023.9840811
TAATACT7350.023.5216714
GTATAAA10650.023.0941681
GTATAAT8900.022.7583641
GTATATA11650.022.3537081
GTGTAAC9100.022.258181
GTATTAG9800.022.1446171
Produced by FastQC (version 0.11.5)