FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821845
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC24000.036.218471
GTCTCGC10700.034.8161541
TCTGTCG11850.032.1960568
TCGCTCT12600.030.8536724
CTGTCGC14250.027.7853629
GTATTAC6050.027.7694241
GCGTTAC1606.492404E-527.3921221
GTATAAG8100.027.0539461
TAATACT8650.024.968294
GTCTTAC10700.024.5761091
GTCGTAA1500.001431063324.348551
GTTAGCC14950.023.5951713
TCTCGCT14400.023.498192
CGCTCTG14450.022.4192245
CTCGCTC15150.021.858153
CTTGCTC34300.021.8276753
ATACTGA12350.021.5676616
GTATTGG8600.021.2342011
GAGTGCA65850.020.8807376
TCTTGCT38050.020.8131142
Produced by FastQC (version 0.11.5)