FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837572
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8050.048.5092051
TCGCTCT9100.042.7426034
TCTGTCG8050.042.033728
CGCTCTG9900.038.54435
CTGTCGC9200.036.77959
TCTCGCT10850.036.5284082
CTCGCTC10700.036.3541533
GTCTTGC18250.036.0582661
GTATTAG6550.034.2252541
TAATACT5450.031.7192784
GTATTAC4050.030.3542961
GTGTTAC8550.029.602491
GTATAGA5800.028.67651
GTTTCAC26500.026.9112193
TACACTG12350.025.0680735
GGGTTTC31250.024.9919431
GTGTATA9600.024.8581621
TTACACT10200.024.7159024
TCTGTCA26000.024.6440728
GGTTTCA27700.024.1936262
Produced by FastQC (version 0.11.5)