FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837572
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7900.042.5294531
TCTGTCG7550.041.9523288
TCGCTCT9800.038.931414
GTACGGA1604.3028194E-836.5198551
TACACCG1251.3288178E-534.5534635
TCTCGCT10700.034.3150022
GTCTTGC16750.033.5764661
CGCTCTG10800.033.3270265
CTGTCGC9550.033.168319
CTCGCTC11200.031.4940433
GTATTAC4500.030.8389911
GGGTACG1855.493468E-627.6366481
GTATTAA5350.027.3045671
GTATAAT4550.027.2895641
GTGTAAA12850.026.7149161
GTATAGG5900.025.9971871
GTTTCAC27650.025.774513
GGGTTTC29850.025.2029841
TACACTA4101.8189894E-1224.5807155
GTACTAT4500.024.3465711
Produced by FastQC (version 0.11.5)