FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800308
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13150.046.538378
CTGTCGC15350.040.8064549
TCGCTCT14000.030.8555624
GTCTCGC14300.030.7144551
GTCTTGC26300.029.0201151
TCTCGCT17150.026.4499862
CTCGCTC17800.025.4841163
GTATTAT8200.025.4643841
GTCTCAC35650.025.4483741
GTACGGT1701.0813469E-425.4127331
GTGTATA16700.024.5757881
GTGTTAG20800.023.8855251
GTATTAG10450.023.426731
TTACACT14650.023.0977924
CGCTCTG17550.022.973185
GTCTTAC10350.022.9573961
GTATAAA12300.022.8301371
GTGTTAC13200.022.3644891
TCACTCT42500.021.5141934
GGGTTTC49600.021.3395211
Produced by FastQC (version 0.11.5)