FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788028
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16950.039.9280858
GTCTCGC16350.037.87451
TCGCTCT17100.035.3669664
CTGTCGC19350.034.6036839
GTCTTGC36350.030.7038961
TCTCGCT19550.030.5701472
GTATTAC10850.028.5367771
CGCTCTG21650.026.9368925
GTATAAT11900.026.6239171
CTCGCTC21350.026.3064923
GTATAGA11150.023.8942321
TTGCTCT51100.022.9657884
GTCTTAC16200.022.6684271
GTGTAAA22500.022.401741
GTGTTAG28100.022.2935471
GGGTTTC64650.022.275511
GTGTATA21950.021.9789281
GTATTCG3004.8723996E-721.6016771
TACACTG25200.021.427975
GTATAGT10850.021.2366711
Produced by FastQC (version 0.11.5)