FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788028
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17200.039.343228
GTCTCGC16350.034.7840231
CTGTCGC22500.032.3153849
GTACTAT9100.031.6438311
GTCTTGC37750.031.2750031
TAATACT11950.030.1204244
GTATTAT11000.026.832531
TCGCTCT20150.026.0799524
CGCTCTG20750.025.6727645
GTATTAA12200.025.3734251
TCTCGCT21400.024.8936392
GTGTTAG26200.024.1797521
GTATAAC7650.023.5260521
GTATTAG13200.023.4511971
GTCTTAC16550.022.6191251
CTCGCTC23050.022.4864353
GAACCGT3854.6929927E-1022.4383546
GTATAAG10650.022.3066751
TTGCTCT51050.022.2802644
TAGTACT9250.021.7909074
Produced by FastQC (version 0.11.5)