FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523331
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15450.041.4787751
TCTGTCG15150.040.8702358
GTCTTGC33050.032.4622961
TCGCTCT17100.032.4197544
CTGTCGC20000.030.239229
TCTCGCT20200.029.5863322
CTCGCTC20650.027.8955143
GTGTACG4300.026.7926651
GTACTAT8600.025.9553951
CGCTCTG19750.025.5179675
GTATTAG12000.025.2018531
GTATAAT9450.025.1447051
GTCTCAC42100.025.141991
ACCGTGT10650.025.0134378
GTGTATA19650.024.5514241
GTCTTAC11750.024.512441
GTGTTAG24300.024.2980821
GTACCGC2401.6982212E-624.0017661
GTATTAT10000.023.7617471
GGGTTTC60550.023.4269921
Produced by FastQC (version 0.11.5)