FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3098435
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12800.045.5609638
GTCTCGC15950.038.3741841
CTGTCGC17700.033.354819
TCGCTCT17450.032.1822934
GTCTTGC27600.031.8296361
TCTCGCT18900.030.0985952
GTACCGA1607.156819E-527.0030121
CTCGCTC19650.026.3846233
TATAGCG1658.8321576E-526.1809355
GTATTAG10900.025.7643411
GTATTGA10000.025.2028081
CGCTCTG20150.025.0115645
GTGTTAG21900.024.0026741
GTCTTAC10850.023.8920651
GTACTAT7000.023.659781
GTATTAA7950.023.5497971
GTCTCAC36200.023.4722291
GGTATAC6800.023.2967171
ACCGTGT10950.023.0129228
GGGTTTC49550.022.961191
Produced by FastQC (version 0.11.5)