FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3098435
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12300.040.9716871
TCTGTCG12200.038.9433178
TCGCTCT14150.035.6126634
GTCTTGC27550.033.1873861
CTGTCGC17250.031.2983239
TCTCGCT16000.031.0470282
CGCTCTG17050.029.133165
GGTATAC7200.028.9972251
CTCGCTC18300.027.93043
GTGTTAC12850.026.8923681
GTATAGG7100.026.3636741
GTATAAT8750.025.5061321
GTCTCAC36750.025.0751511
GTGTTAG21850.024.7115941
GTTAGCC17000.023.714233
GTCCTAC9350.023.0993941
GTGTTAA12700.022.6749951
GGAGTGC72650.022.3942095
GAGTGCA77800.022.3001276
TTGCTCT39100.022.277764
Produced by FastQC (version 0.11.5)