FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4474637
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17950.037.303098
GTCTCGC20400.036.7071
GTCTTGC44150.033.4325031
CTGTCGC21950.032.4733439
TCGCTCT21750.030.785084
TCTCGCT23950.027.9590972
GTATTAT14050.027.1609671
GTATTAA13800.027.131261
CTCGCTC24600.025.7568823
GTGTTAC19350.025.67521
GTATAGT12450.025.4465561
CGCTCTG26850.025.2060625
TTCACCG13050.024.8269545
TTGCTCT61250.024.0970754
GTCTTAC18700.023.8723851
GTATTAG16600.023.42241
GTGTAAC16000.023.4007111
GTATTAC11800.023.1871471
TTACACT21350.022.594024
TACACTG29300.022.1154775
Produced by FastQC (version 0.11.5)