FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4231961
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18900.043.8030858
GTCTCGC20000.035.2773171
CTGTCGC24700.034.6831639
GTCTTGC39850.034.5067861
TCGCTCT22650.032.1012424
TCTCGCT24400.027.4405382
CTCGCTC24450.027.3824833
CGCTCTG24700.025.6480545
TACTTCG2259.698888E-725.5962417
GTCTCAC52500.024.5467071
GTATTAA12650.023.903321
GTGTAAA28400.023.5756721
TTGCTCT54900.023.4719624
GTGTTAT17200.023.4400791
GGTATAC8000.022.498291
GTTTCAC62700.022.3890553
GTATTAT11700.022.1521641
GGGTTTC67950.022.0380611
GTGTTAA18700.021.9448551
TCACTCT62200.021.8746014
Produced by FastQC (version 0.11.5)