FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265851
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16850.037.6025478
GTCTCGC16850.036.3206441
CTGTCGC21150.034.042719
TCGCTCT19200.031.8736534
GTATTAG14750.029.288271
TCTCGCT21200.027.8491862
GTCTTGC37750.026.892841
GTCTTAC17700.025.627241
CGTTTCG2001.1252927E-525.2001179
CTCGCTC22050.025.1429753
CGCTCTG23750.024.5556165
GTATTAC9700.024.494961
GGTATAC9650.023.8757571
GTGTTAC18650.023.5497071
TAGTACT9900.023.2717444
GTGTTAG29450.022.9814321
GTTTCAC68000.022.8706933
TAATACT13550.022.8477444
GGGTTTC73150.022.7369481
GTATTGC12100.022.6116751
Produced by FastQC (version 0.11.5)