FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265851
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC19000.044.7086261
TCTGTCG19000.040.161528
TCGCTCT21050.036.2494474
TCGATCG800.0021097735.9929664
CTGTCGC24700.033.5165029
TCTCGCT25000.031.6749252
CGCTCTG23050.030.9180375
GTCTTGC41800.030.4831541
CTCGCTC25650.028.6259863
GGTATAC9500.025.7642921
GTGTTAG29900.025.2802311
GTATTAT12500.024.1881241
TTGCTCT55000.023.2972664
GTACTAT9950.023.1520711
GTATAGT12700.022.6735321
GTCTTAC18800.022.2092061
GTCTCAC53600.021.6234021
TACACTG26100.021.5130375
TCTGTCA56700.020.8218158
CTTGCTC54500.020.6051483
Produced by FastQC (version 0.11.5)