FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3958770
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC17600.032.3208121
TCTGTCG15450.031.68998
GTCTTGC37350.028.9180181
TCGCTCT19600.027.550134
GTATTAA13750.027.2313061
CTGTCGC19350.026.0469869
GTATAGA12300.025.1727811
GTACTAT11000.024.8747541
GTGTAGG24700.024.7793451
GTCTTAC17950.024.4699571
TCTCGCT22400.024.4305612
CTCGCTC22400.023.4661963
GTGTATA21100.023.205681
CGCTCTG22150.023.0788465
CTATGCG2903.517489E-722.3452369
GTGTTAC17650.022.0301231
GTATAAC7800.021.2324981
GTGTAGA23650.021.008051
GTATTAC9700.020.7851981
GTGTTAG27300.020.5731031
Produced by FastQC (version 0.11.5)