FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3958770
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17500.042.3715828
CTGTCGC22550.032.2441029
GTCTCGC17450.029.7045881
TCGCTCT19750.028.0675584
GTCTTGC38350.027.970961
GTATTAG13850.027.5494291
TACGCTA1400.001041906425.7112375
GGTATAC7950.025.3558031
ATACTAT14100.022.465426
TCTCGCT22850.022.369622
CGCTCTG22550.022.3476815
CTCGCTC23250.022.294133
GTACTAT9700.022.265681
TATAACG2303.320319E-521.9107232
GTATAAT11750.021.4445361
GTATAGG9500.021.2188011
GTATCGA1700.00322332121.173966
GTATAGT11250.021.117761
GTCTCAC48800.020.8010731
AGTACCG3157.803101E-720.5689895
Produced by FastQC (version 0.11.5)