FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3717166
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16350.036.5448658
TCGCTCT16400.030.2872354
GTCTCGC17100.029.8901631
GTCTTGC33450.029.0538481
CTGTCGC21200.028.5239379
GTACTAT10200.026.1136511
GTATAAG12450.024.285441
GTGTTAG24650.024.2396981
CGCTCTG20400.023.6428035
TAGTACT8850.023.5889764
CTCGCTC19650.023.4461613
TCTCGCT20150.023.2222542
GTATTAC8850.022.7761751
GTGTTAC16400.021.9478631
GCGTAAC2353.9208247E-521.4435271
GTCTTAC15450.021.4336131
TTGCTCT50950.021.193444
GTTCTAC12250.021.1559451
GTCTCAC46800.021.0736981
GTGTATA22050.020.5682811
Produced by FastQC (version 0.11.5)