FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999487
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8300.034.697928
GTCTCGC7950.028.9854771
GTGTTAG13250.028.8043171
GTCTTGC17250.028.8043171
GTATTAG7250.028.8043141
TCGCTCT8350.027.592124
GTATACG1100.00999838626.1857431
GGTATAC3850.026.1857411
AGTACGA1450.001277559724.8269622
CTGTCGC10850.024.5523769
GTCTTAC7350.024.4934671
GTTCCGA2101.6398264E-524.00361
GTACTAG4303.6379788E-1223.4453741
CTCGCTC9850.023.3902743
CTCGATC8250.022.6903368
GTACAGA11700.022.1571671
GTAGTAC3956.4392225E-1021.8728683
TATTAGG6300.021.713742
GTTAGCC11300.021.6631723
GGATAAT9000.021.6032371
Produced by FastQC (version 0.11.5)