FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165092
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8950.041.83148
GGTTACG1201.0003809E-536.0157741
GTCTCGC10000.033.8548281
CTGTCGC12250.032.9134989
TCGCTCT10650.029.069734
GTCTTGC18450.028.109871
TCTCGCT12050.026.899752
GTATTAG7850.026.6103821
CGCTCTG11950.025.907335
CTCGCTC12050.025.701833
GTATTAA6300.025.1538751
GTGTTAG16050.025.1325021
GTGTAAC6800.023.3043251
GTGTTAC9350.023.1117271
GTGTAGC10100.022.1086941
TAATACT7550.021.933254
GGGTTTC38150.021.9021241
GTTTCAC34600.021.8572653
GTCTTAC9650.021.6467881
TTGCTCT27450.021.2453944
Produced by FastQC (version 0.11.5)