FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165092
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.037.112958
CTGTCGC11350.033.6207359
GTCTCGC9600.033.007981
TATACGG1102.5369256E-432.7298972
GTCTTGC20650.032.4339941
GTGTAAC6900.029.2244591
GTATAGG5800.028.5586321
TCGCTCT11800.027.456664
CGTTTCG1358.4198744E-426.6663429
TAACGGT1358.4210106E-426.6657284
GTGTTAG15900.026.2705061
TCTCGCT12900.026.2346632
CTCGCTC12500.025.9190853
GTATTAT6950.025.9055461
GTGTTAC8200.024.5913121
GTCTCAC24350.024.548031
GTTAGCC11450.024.5231513
CGCTCTG12250.023.5099165
GTCTTAC9300.023.2314241
GTGTAGG13750.022.521811
Produced by FastQC (version 0.11.5)